Apc^tm2.1Cip
Targeted Allele Detail

Summary

• Symbol: Apc^tm2.1Cip
• Name: APC, WNT signaling pathway regulator; targeted mutation 2, Christine Perret
• MGI ID: MGI:3521822
• Synonyms: Apc^2lox14, Apc^fle14, Apc^lox, Apc^tm2Cpe
• Gene: Apc Location: Chr18:34353977-34455605 bp, + strand Genetic Position: Chr18, 18.53 cM
• Alliance: Apc^tm2.1Cip page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94721
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into intron 13 and a loxP flanked PGK-Hprt cassette into intron 14. Cre mediated recombination removed a floxed HPRT cassette that was inserted into exon 14, leaving exon 14 flanked with loxP sites. (J:94721)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)

	Allelic Composition	Genetic Background	Cell Line(s)
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cn1  Disease Model	Apc^tm2.1Cip/Apc^tm2.1Cip	involves: 129P2/OlaHsd * C57BL/6N
cn2	Apc^tm2.1Cip/Apc^tm2.1Cip Tg(Ttr-cre/Esr1*)1Vco/0	B6.Cg-Apc^tm2.1Cip Tg(Ttr-cre/Esr1*)1Vco
cn3	Apc^tm2.1Cip/Apc^tm2.1Cip Lect2^tm1Ymg/Lect2^tm1Ymg Tg(Ttr-cre/Esr1*)1Vco/0	B6.Cg-Lect2^tm1Ymg Apc^tm2.1Cip Tg(Ttr-cre/Esr1*)1Vco
cn4	Apc^tm2.1Cip/Apc^+ Lrig1^tm1.1(cre/ERT2)Rjc/Lrig1^+	involves: 129 * 129P2/OlaHsd * C57BL/6
cn5	Apc^tm2.1Cip/Apc^+ Kras^tm4Tyj/Kras^+ Tg(Fabp1-cre)1Jig/0	involves: 129P2/OlaHsd * 129S4/SvJae * FVB
cn6	Apc^tm2.1Cip/Apc^+ Lgr5^tm1(cre/ERT2)Cle/Lgr5^+	involves: 129P2/OlaHsd * C57BL/6
cn7	Apc^tm2.1Cip/Apc^+ Atg7^tm1Tchi/Atg7^tm1Tchi Tg(Vil1-cre/ERT2)23Syr/0	involves: 129P2/OlaHsd * C57BL/6 * C57BL/6NCrlj * CBA/JNCrlj * DBA/2
cn8	Apc^tm2.1Cip/Apc^+ Tg(Vil1-cre)20Syr/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2
cn9  Disease Model	Apc^tm2.1Cip/Apc^+ Tg(Vil1-cre/ERT2)23Syr/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2
cn10	Apc^tm2.1Cip/Apc^tm2.1Cip Tg(Vil1-cre/ERT2)23Syr/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2
cn11	Apc^tm2.1Cip/Apc^+ Tg(Fabp1-cre)1Jig/0	involves: 129P2/OlaHsd * FVB/N

Phenotypes:

Affected Systems	cn1	cn2	cn3	cn4	cn5	cn6	cn7	cn8	cn9	cn10	cn11
show or hide all annotated terms
cellular			√				√		√	√
abnormal intestinal goblet cell morphology							√			√
abnormal autophagy									√
increased hepatocyte apoptosis			√
increased small intestinal crypt cell apoptosis										√
digestive/alimentary system				√	√		√	√	√	√	√
abnormal intestinal goblet cell morphology							√			√
abnormal intestine development										√
abnormal intestinal epithelium morphology										√
abnormal small intestine crypts of Lieberkuhn morphology										√
abnormal Paneth cell morphology							√
abnormal colon morphology										√
rectal prolapse									√
decreased intestinal adenoma incidence							√
increased intestinal adenocarcinoma incidence					√						√
increased intestinal adenoma incidence				√				√	√
increased colon adenoma incidence								√	√
intestine polyps									√
abnormal gut flora balance							√		√
abnormal intestine physiology							√
increased small intestinal crypt cell apoptosis										√
endocrine/exocrine glands							√			√
abnormal small intestine crypts of Lieberkuhn morphology										√
abnormal Paneth cell morphology							√
growth/size/body	√	√							√
cachexia									√
enlarged liver		√
liver hyperplasia	√
hematopoietic system		√	√				√
increased CD103-positive CD11b-low dendritic cell number							√
increased CD8-positive, alpha-beta T cell number							√
decreased NK T cell number		√
increased NK T cell number			√
increased regulatory T cell number							√
abnormal neutrophil physiology			√
abnormal NK T cell physiology		√	√
homeostasis/metabolism			√						√
abnormal autophagy									√
abnormal circulating enzyme level			√
abnormal chemokine level			√
immune system		√	√				√
increased CD103-positive CD11b-low dendritic cell number							√
increased CD8-positive, alpha-beta T cell number							√
decreased NK T cell number		√
increased NK T cell number			√
increased regulatory T cell number							√
abnormal immune system physiology							√
abnormal neutrophil physiology			√
abnormal NK T cell physiology		√	√
abnormal chemokine level			√
decreased interferon-gamma secretion			√
increased interferon-gamma secretion		√
increased interleukin-1 beta secretion							√
increased interleukin-10 secretion							√
increased interleukin-4 secretion		√	√
liver inflammation		√	√
liver/biliary system	√	√	√
increased hepatocyte apoptosis			√
liver inflammation		√	√
abnormal liver morphology		√	√
enlarged liver		√
liver hyperplasia	√
hepatic necrosis			√
increased hepatocellular carcinoma incidence	√
mortality/aging	√		√		√				√	√
premature death	√		√		√					√
decreased tumor-free survival time									√
neoplasm	√			√	√	N	√	√	√		√
neoplasm						N
decreased intestinal adenoma incidence							√
increased intestinal adenocarcinoma incidence					√						√
increased intestinal adenoma incidence				√				√	√
increased colon adenoma incidence								√	√
increased hepatocellular carcinoma incidence	√

View phenotypes and curated references for all genotypes (concatenated display).

Disease models

Key:

√

disease model

expected model not found

Models:

Human Diseases	cn1	cn9
colorectal cancer IDs colorectal cancer       DOID:9256 ICD10CM:C18.9 KEGG:05210 MESH:D015179 NCI:C2956 NCI:C4978 OMIM:114500 UMLS_CUI:C0009404 UMLS_CUI:C0346629 Close		√
hepatocellular carcinoma IDs hepatocellular carcinoma       DOID:684 DOID:5005 EFO:0000182 ICD10CM:C22.0 MESH:D006528 NCI:C3099 OMIM:114550 ORDO:88673 UMLS_CUI:C2239176 Close	√

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	7 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Apc^tm2.1Cip

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Apc Mutation: 157 strains or lines available

References

• Original: J:94721 Colnot S, et al., Liver-targeted disruption of Apc in mice activates beta-catenin signaling and leads to hepatocellular carcinomas. Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):17216-21
• All: 37 reference(s)