Phox2a^{tm1(Phox2b)Mist}
Targeted Allele Detail

Summary

• Symbol: Phox2a^{tm1(Phox2b)Mist}
• Name: paired-like homeobox 2a; targeted mutation 1, Michele Studer
• MGI ID: MGI:3521825
• Synonyms: Phox2a^KI2b
• Gene: Phox2a Location: Chr7:101467520-101471933 bp, + strand Genetic Position: Chr7, 54.66 cM
• Alliance: Phox2a^{tm1(Phox2b)Mist} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126634
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The Phox2b coding sequence was inserted upstream of exon 1 along with a floxed neo cassette which was later removed by cre-mediated recombination following a cross to Tg(ACTB-cre)1Nagy mice. The insertion creates a fusion protein. Southern blot analysis and PCR genotyping were used to confirm the insertion. Immunohistochemistry was used to confirm the alteration in expression patterns. (J:126634)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Phox2a Mutation: 10 strains or lines available

References

• Original: J:126634 Coppola E, et al., Reciprocal gene replacements reveal unique functions for Phox2 genes during neural differentiation. EMBO J. 2005 Dec 21;24(24):4392-403
• All: 1 reference(s)