Phox2b^{tm1(Phox2a)Mist}
Targeted Allele Detail

Summary

• Symbol: Phox2b^{tm1(Phox2a)Mist}
• Name: paired-like homeobox 2b; targeted mutation 1, Michele Studer
• MGI ID: MGI:3521828
• Synonyms: Phox2b^KI2a
• Gene: Phox2b Location: Chr5:67251751-67256399 bp, - strand Genetic Position: Chr5, 35.95 cM
• Alliance: Phox2b^{tm1(Phox2a)Mist} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126634
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Targeting took place in PC3 ES cells homozygous for Tg(Prm-cre)70Og, a cre transgene that expresseses Cre recombinase only in halpoid spermatids under control of the male-germline specific protamine promoter. The Phox2a coding sequence was inserted upstream of exon 1 along with an IRES-GFP and a floxed neo cassette, which was removed by cre-mediated recombination upon passage through the male germline. In situ hybridization and immunohistochemistry confirm the altered expression pattern of the knocked in coding region and absence of endogenous target gene expression. For unknown reasons the EGFP reporter was nonfunctional. (J:126634)

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Phox2b Mutation: 25 strains or lines available

References

• Original: J:126634 Coppola E, et al., Reciprocal gene replacements reveal unique functions for Phox2 genes during neural differentiation. EMBO J. 2005 Dec 21;24(24):4392-403
• All: 2 reference(s)