Top2b^tm2Jcw
Targeted Allele Detail

Summary

• Symbol: Top2b^tm2Jcw
• Name: topoisomerase (DNA) II beta; targeted mutation 2, James C Wang
• MGI ID: MGI:3521850
• Synonyms: top2beta^flox1, top2beta^flox2
• Gene: Top2b Location: Chr14:6038976-6104585 bp, - strand Genetic Position: Chr14, 7.08 cM, cytoband A3
• Alliance: Top2b^tm2Jcw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94879
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A self excising neomycin resistance cassette together with an upstream loxP site was inserted in one intron (4?) and a second loxP site was inserted in the third downstream exon. The presence or absence of the neomycin cassette was assessed by PCR but did not affect selection of mice for breeding since the resistance cassette was contained between the two loxP sites. (J:94879)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	12 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Top2b Mutation: 91 strains or lines available

References

• Original: J:94879 Lyu YL, et al., Aberrant lamination in the cerebral cortex of mouse embryos lacking DNA topoisomerase IIbeta. Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7123-8
• All: 8 reference(s)