Mmp13^tm1Smk
Targeted Allele Detail

Summary

• Symbol: Mmp13^tm1Smk
• Name: matrix metallopeptidase 13; targeted mutation 1, Stephen M Krane
• MGI ID: MGI:3521852
• Synonyms: Mmp-13^-, Mmp13^-
• Gene: Mmp13 Location: Chr9:7272514-7283331 bp, + strand Genetic Position: Chr9, 2.46 cM, cytoband A1-A2
• Alliance: Mmp13^tm1Smk page

Long bone analysis from wild type and Mmp13^tm1Smk/Mmp13^tm1Smk mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94723
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a PGK-neo and a stop codon in order to splice out exon 5, which encodes the zinc-binding residues in the catalytic domain. Northern blot of mutant calvariae demonstrated a lack of transcript when an exon 5 probe was used and a barely detectable amount of transcript when using a 5' probe. Western blot showed no protein in cultures of mutant calvariae. (J:94723)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mmp13 Mutation: 36 strains or lines available

References

• Original: J:94723 Inada M, et al., Critical roles for collagenase-3 (Mmp13) in development of growth plate cartilage and in endochondral ossification. Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):17192-7
• All: 18 reference(s)