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Ptk2tm1Gwm
Targeted Allele Detail
Summary
Symbol: Ptk2tm1Gwm
Name: PTK2 protein tyrosine kinase 2; targeted mutation 1, Gordon W McLean
MGI ID: MGI:3521998
Synonyms: FAKflox
Gene: Ptk2  Location: Chr15:73076951-73295129 bp, - strand  Genetic Position: Chr15, 33.94 cM
Alliance: Ptk2tm1Gwm page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:94680
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA targeting vector was designed to introduce loxP sites to flank the exon encoding part of the kinase domain, encoded by amino acids 413-444. Upon transfection with a Cre-expressing plasmid, the floxed PGK-neo cassette was deleted, leaving the exon floxed. The targeting vector was designed so that Cre-mediated recombination would introduce a frameshift was created in the adjacent exon, preventing production of a functional protein. Immunoblot confirmed the lack of wild-type protein or truncated protein created by this allele in Cre-expressing ES cells. (J:94680)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ptk2 Mutation:  91 strains or lines available
References
Original:  J:94680 McLean GW, et al., Specific deletion of focal adhesion kinase suppresses tumor formation and blocks malignant progression. Genes Dev. 2004 Dec 15;18(24):2998-3003
All:  22 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory