Ptk2tm1Gwm
Targeted Allele Detail
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Symbol: |
Ptk2tm1Gwm |
Name: |
PTK2 protein tyrosine kinase 2; targeted mutation 1, Gordon W McLean |
MGI ID: |
MGI:3521998 |
Synonyms: |
FAKflox |
Gene: |
Ptk2 Location: Chr15:73076951-73295129 bp, - strand Genetic Position: Chr15, 33.94 cM
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Alliance: |
Ptk2tm1Gwm page
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Germline Transmission: |
Earliest citation of germline transmission:
J:94680
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Parent Cell Line: |
E14TG2a (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Conditional ready, No functional change) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A targeting vector was designed to introduce loxP sites to flank the exon encoding part of the kinase domain, encoded by amino acids 413-444. Upon transfection with a Cre-expressing plasmid, the floxed PGK-neo cassette was deleted, leaving the exon floxed. The targeting vector was designed so that Cre-mediated recombination would introduce a frameshift was created in the adjacent exon, preventing production of a functional protein. Immunoblot confirmed the lack of wild-type protein or truncated protein created by this allele in Cre-expressing ES cells.
(J:94680)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Ptk2 Mutation: |
91 strains or lines available
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Original: |
J:94680 McLean GW, et al., Specific deletion of focal adhesion kinase suppresses tumor formation and blocks malignant progression. Genes Dev. 2004 Dec 15;18(24):2998-3003 |
All: |
22 reference(s) |
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