About   Help   FAQ
Ptk2tm1Gwm
Targeted Allele Detail
Summary
Symbol: Ptk2tm1Gwm
Name: PTK2 protein tyrosine kinase 2; targeted mutation 1, Gordon W McLean
MGI ID: MGI:3521998
Synonyms: FAKflox
Gene: Ptk2  Location: Chr15:73076951-73295129 bp, - strand  Genetic Position: Chr15, 33.94 cM
Alliance: Ptk2tm1Gwm page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:94680
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA targeting vector was designed to introduce loxP sites to flank the exon encoding part of the kinase domain, encoded by amino acids 413-444. Upon transfection with a Cre-expressing plasmid, the floxed PGK-neo cassette was deleted, leaving the exon floxed. The targeting vector was designed so that Cre-mediated recombination would introduce a frameshift was created in the adjacent exon, preventing production of a functional protein. Immunoblot confirmed the lack of wild-type protein or truncated protein created by this allele in Cre-expressing ES cells. (J:94680)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ptk2 Mutation:  91 strains or lines available
References
Original:  J:94680 McLean GW, et al., Specific deletion of focal adhesion kinase suppresses tumor formation and blocks malignant progression. Genes Dev. 2004 Dec 15;18(24):2998-3003
All:  22 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
10/29/2024
MGI 6.24
The Jackson Laboratory