Gapdhs^tm1Dao
Targeted Allele Detail

Summary

• Symbol: Gapdhs^tm1Dao
• Name: glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; targeted mutation 1, Deborah A O'Brien
• MGI ID: MGI:3522094
• Synonyms: Gapds^-
• Gene: Gapdhs Location: Chr7:30429204-30443106 bp, - strand Genetic Position: Chr7, 19.23 cM, cytoband A3
• Alliance: Gapdhs^tm1Dao page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94734
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 5 and part of exon 6 were replaced with a neo cassette. Western blot showed the absence of protein in mutant testis and sperm. (J:94734)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gapdhs Mutation: 16 strains or lines available

References

• Original: J:94734 Miki K, et al., Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility. Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16501-6
• All: 5 reference(s)