Adam10^tm1Psa
Targeted Allele Detail

Summary

• Symbol: Adam10^tm1Psa
• Name: a disintegrin and metallopeptidase domain 10; targeted mutation 1, Paul Saftig
• MGI ID: MGI:3522353
• Synonyms: Adam10^-
• Gene: Adam10 Location: Chr9:70586279-70687511 bp, + strand Genetic Position: Chr9, 39.53 cM
• Alliance: Adam10^tm1Psa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94943
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The 5' exon was interrupted by the insertion of a neomycin expression cassette. This introduces a premature stop codon into the ORF. RT-PCR and Northern blot analysis confirmed the absence of transcription. Western blot analysis demonstrated the absence of a protein product. (J:94943)

Expression

In Mice Carrying this Mutation:	8 assay results
In Structures Affected by this Mutation:	9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adam10 Mutation: 38 strains or lines available

References

• Original: J:94943 Hartmann D, et al., The disintegrin/metalloprotease ADAM 10 is essential for Notch signalling but not for alpha-secretase activity in fibroblasts. Hum Mol Genet. 2002 Oct 1;11(21):2615-24
• All: 12 reference(s)