Macroh2a1^tm1Peh
Targeted Allele Detail

Summary

• Symbol: Macroh2a1^tm1Peh
• Name: macroH2A.1 histone; targeted mutation 1, John R Pehrson
• MGI ID: MGI:3522572
• Synonyms: macroH2A1^-
• Gene: Macroh2a1 Location: Chr13:56221435-56283439 bp, - strand Genetic Position: Chr13, 30.06 cM, cytoband B2
• Alliance: Macroh2a1^tm1Peh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:121382
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An IRESBgeo cassette was inserted in 1st intron. This cassette was flanked by LoxP sites and a 3rd loxP site was inserted in the 2nd intron. Transient cre expression removed the floxed sequence, including exon 2, which encodes the first part of the H2A domain. Western blot analysis confirmed the absence of protein product. (J:121382)

Expression

In Mice Carrying this Mutation:

35 assay results 2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Macroh2a1 Mutation: 50 strains or lines available

Notes

ES cell line = 98-2 (Incyte Genomics; "129svJ").

References

• Original: J:121382 Changolkar LN, et al., Developmental changes in histone macroH2A1-mediated gene regulation. Mol Cell Biol. 2007 Apr;27(7):2758-64
• All: 8 reference(s)