Kcnn2^tm2Jpad
Targeted Allele Detail

Summary

• Symbol: Kcnn2^tm2Jpad
• Name: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2; targeted mutation 2, John P Adelman
• MGI ID: MGI:3522587
• Synonyms: mSK2 tTAdeltaneo, SK2T
• Gene: Kcnn2 Location: Chr18:45401927-45818950 bp, + strand Genetic Position: Chr18, 24.34 cM, cytoband C
• Alliance: Kcnn2^tm2Jpad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:105645
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A genetic switch based on the tetracycline transactivator/tetracycline operator (tTA/tetO) system was inserted into the 5' untranslated region (5' UTR) of the targeted gene 40 nucleotides upstream of the initiator ATG. The switch, after in vivo excision of a selection cassette, comprises a tripartite adenovirus leader sequence (to enhance translation initiation efficiency) followed by the coding sequence for the tTA protein with SV40 and human growth hormone polyadenylation signals separated by a single loxP site; and five copies of the tetracycline operator (tetO), also called the tetracycline responsive element (TRE), joined to the cytomegalovirus (CMV) minimal promoter. In the absence of doxycycline (Dox), heterozygous mutant mice express 4.5-fold the wild-type level of Kcnn2 mRNA and 10-fold the wild-type level of Kcnn2 subunit protein, respectively assessed by quantitative and real-time PCR of whole-brain RNA and by immunoblot analysis of membrane proteins from whole brains. Administration of Dox reduces, but does not eliminate, expression from the mutant allele. (J:95064, J:105645)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kcnn2 Mutation: 43 strains or lines available

References

• Original: J:105645 Hammond RS, et al., Small-conductance Ca2+-activated K+ channel type 2 (SK2) modulates hippocampal learning, memory, and synaptic plasticity. J Neurosci. 2006 Feb 8;26(6):1844-53
• All: 7 reference(s)