Aph1a^tm1Bdes
Targeted Allele Detail

Summary

• Symbol: Aph1a^tm1Bdes
• Name: aph1 homolog A, gamma secretase subunit; targeted mutation 1, Bart De Strooper
• MGI ID: MGI:3522625
• Synonyms: Aph1A^flx
• Gene: Aph1a Location: Chr3:95801281-95805600 bp, + strand Genetic Position: Chr3, 41.47 cM
• Alliance: Aph1a^tm1Bdes page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:96106
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: FRT-flanked PGK-hygro with a loxP site downstream and a lacZ sequence was inserted downstream of the gene in position 3444. A second loxP site was inserted into position 540 in intron 1. RT-PCR and Western blot of mutant MEFs demonstrated the expression of mRNA or protein, respectively. (J:96106)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Aph1a Mutation: 19 strains or lines available

References

• Original: J:96106 Serneels L, et al., Differential contribution of the three Aph1 genes to gamma-secretase activity in vivo. Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1719-24
• All: 5 reference(s)