Aph1b^tm1Bdes
Targeted Allele Detail

Summary

• Symbol: Aph1b^tm1Bdes
• Name: aph1 homolog B, gamma secretase subunit; targeted mutation 1, Bart De Strooper
• MGI ID: MGI:3522628
• Synonyms: Aph1b^-
• Gene: Aph1b Location: Chr9:66682484-66702772 bp, - strand Genetic Position: Chr9, 36.12 cM, cytoband D
• Alliance: Aph1b^tm1Bdes page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:96106
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a modified human placental alkaline phosphatase reporter sequence, polyA signal at the ATG in exon 1. RT-PCR and Western blot of mutant MEFs demonstrated the expression of mRNA or protein, respectively. (J:96106)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Aph1b Mutation: 19 strains or lines available

References

• Original: J:96106 Serneels L, et al., Differential contribution of the three Aph1 genes to gamma-secretase activity in vivo. Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1719-24
• All: 7 reference(s)