Kcnn3^tm1Jpad
Targeted Allele Detail

Summary

• Symbol: Kcnn3^tm1Jpad
• Name: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3; targeted mutation 1, John P Adelman
• MGI ID: MGI:3522659
• Synonyms: KCa2.3^T, SK3^T
• Gene: Kcnn3 Location: Chr3:89427471-89579801 bp, + strand Genetic Position: Chr3, 39.16 cM, cytoband F2
• Alliance: Kcnn3^tm1Jpad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95064
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Insertion
• Mutation details: A tet casette, containing tTA protein coding sequence, floxed neo coding sequence and tet operator/CMV promoter, was inserted into the 5' UTR of the SK3 gene 42 nt 5' of the initiator MET. Administration of doxycycline to homozygous tet anmials results in knockout of SK3 expression. In the absence of tet or dox, SK3 protein is overexpressed 3-5 fold over WT. (J:95064)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kcnn3 Mutation: 51 strains or lines available

References

• Original: J:95064 Bond CT, et al., Respiration and parturition affected by conditional overexpression of the Ca2+-activated K+ channel subunit, SK3. Science. 2000 Sep 15;289(5486):1942-6
• All: 19 reference(s)