Plce1^tm1Tkat
Targeted Allele Detail

Summary

• Symbol: Plce1^tm1Tkat
• Name: phospholipase C, epsilon 1; targeted mutation 1, Tohru Kataoka
• MGI ID: MGI:3525208
• Synonyms: PLCepsilon^-, PLCepsilon^deltaX
• Gene: Plce1 Location: Chr19:38469557-38773474 bp, + strand Genetic Position: Chr19, 33.16 cM
• Alliance: Plce1^tm1Tkat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94952
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neo was inserted to replace the 3' end of one of the four exons encoding the X domain. RT-PCR demonstrated the presence of a shortened transcript in mutant heart samples. Sequence analysis revealed a deletion of another upstream exon, presumably caused by exon skipping upon RNA splicing. The resulting allele contains an in-frame deletion of amino acids 1333 to 1408, corresponding to the amino terminal end of the catalytic X domain. (J:94952, J:97661)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Plce1 Mutation: 111 strains or lines available

References

• Original: J:94952 Bai Y, et al., Crucial role of phospholipase Cepsilon in chemical carcinogen-induced skin tumor development. Cancer Res. 2004 Dec 15;64(24):8808-10
• All: 10 reference(s)