Ccn6^tm2Mawa
Targeted Allele Detail

Summary

• Symbol: Ccn6^tm2Mawa
• Name: cellular communication network factor 6; targeted mutation 2, Matthew L Warman
• MGI ID: MGI:3525361
• Synonyms: Wisp3^-, Wisp3^{lacZdeltaex3-5}, Wisp3^tm1Mawa
• Gene: Ccn6 Location: Chr10:39026966-39039790 bp, - strand Genetic Position: Chr10, 20.19 cM
• Alliance: Ccn6^tm2Mawa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94500
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A lacZ-neo was inserted into the Wisp3 locus, replacing exons3-5 and creating a null allele. The allele resulted in a fusion protein that has Beta-gal activity under the endogenous promoter. The Wisp3 protein is truncated immediately after the IGFBP domain, and lacks the vWF, TSP and C-terminal domains. RT-PCR of cartilage from mutants showed a lack of wild-type transcript. Beta-gal activity was confirmed in cells transfected with a vector expressing the Wisp3-lacZ fusion protein. (J:94500)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ccn6 Mutation: 23 strains or lines available

References

• Original: J:94500 Kutz WE, et al., WISP3, the gene responsible for the human skeletal disease progressive pseudorheumatoid dysplasia, is not essential for skeletal function in mice. Mol Cell Biol. 2005 Jan;25(1):414-21
• All: 2 reference(s)