Ap3b1^tm1.1Sms
Targeted Allele Detail

Summary

• Symbol: Ap3b1^tm1.1Sms
• Name: adaptor-related protein complex 3, beta 1 subunit; targeted mutation 1.1, Suzanne L Mansour
• MGI ID: MGI:3525672
• Synonyms: Ap3b1^L
• Gene: Ap3b1 Location: Chr13:94495468-94702825 bp, + strand Genetic Position: Chr13, 49.22 cM
• Alliance: Ap3b1^tm1.1Sms page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66256
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: An IRES-lacZ and a floxed PGKneomycin selection cassette was inserted into the coding region of the gene. Subsequent transient transcription of Cre recombinase removed the neomycin selection cassette. Northern blot analysis on RNA derived from spleen and kidney of homozygous mice confirmed that no detectable transcript was expressed from this allele. (J:66256)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ap3b1 Mutation: 96 strains or lines available

References

• Original: J:66256 Yang W, et al., Defective organellar membrane protein trafficking in Ap3b1-deficient cells. J Cell Sci. 2000 Nov;113(Pt 22):4077-86
• All: 6 reference(s)