Sphk1^tm1Rlp
Targeted Allele Detail

Summary

• Symbol: Sphk1^tm1Rlp
• Name: sphingosine kinase 1; targeted mutation 1, Richard L Proia
• MGI ID: MGI:3526071
• Synonyms: SK1^-, Sphk1^-, SphK1 KO
• Gene: Sphk1 Location: Chr11:116421751-116427501 bp, + strand Genetic Position: Chr11, 81.42 cM, cytoband E2
• Alliance: Sphk1^tm1Rlp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95168
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neo replaced exons 3-5 and the 5' end of exon 6, deleting sequence corresponding to the putative lipid kinase catalytic domain, which contains the conserved domains C1, C2, C3 and part of C4, and a putative nucleotide binding motif. Absence of mRNA was demonstrated in kidney samples by Northern blot and RT-PCR analyses. (J:95168)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Sphk1^tm1Rlp

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Sphk1 Mutation: 29 strains or lines available

References

• Original: J:95168 Allende ML, et al., Mice deficient in sphingosine kinase 1 are rendered lymphopenic by FTY720. J Biol Chem. 2004 Dec 10;279(50):52487-92
• All: 99 reference(s)