Car12^{Tn(sb-T2/GT2/tTA)4563.1Dla}
Transposon induced Allele Detail

Summary

• Symbol: Car12^{Tn(sb-T2/GT2/tTA)4563.1Dla}
• Name: carbonic anhydrase 12; transposon insertion 4563.1, David A Largaespada
• MGI ID: MGI:3526109
• Synonyms: 02A-0001, Car12^{Tn(sb-T2/GT2/tTA)1.1Dla}, Car12^{Tn(sb-T2/GT2/tTA)2.1Dla}
• Gene: Car12 Location: Chr9:66620968-66674127 bp, + strand Genetic Position: Chr9, 36.09 cM
• Alliance: Car12^{Tn(sb-T2/GT2/tTA)4563.1Dla} page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Transposon induced (Inserted expressed sequence)
• Mutation: Transposon insertion
• Mutation details: This mutation consists of an insertion of a Sleeping Beauty transposable element gene trap construct into the first intron of this gene. The Sleeping Beauty transposon construct includes a Carp beta-actin splice acceptor and exon sequences with stop codons in all three reading frames, followed by the EMCV internal ribosome entry site (IRES) and Clontech's Tet-off cDNA, with an SV40 polyadenylation signal, all between the inverted terminal repeats of the transposon (IR/DRs), This mutation was generated by germline mobilization of chromosomally-resident transposons from concatemer line 4563. As a consequence, this particular transposon insertion is not unique in the carrier strain. Multiple other linked insertions may exist with this insertion, although none of the others have been shown to be in genes at the time of submission. (J:117975)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Car12 Mutation: 23 strains or lines available

References

• Original: J:117975 Geurts AM, et al., Conditional gene expression in the mouse using a Sleeping Beauty gene-trap transposon. BMC Biotechnol. 2006;6:30
• All: 1 reference(s)