Fbxw8^{Gt(RRT057)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Fbxw8^{Gt(RRT057)Byg}
• Name: F-box and WD-40 domain protein 8; gene trap RRT057, BayGenomics
• MGI ID: MGI:3526146
• Synonyms: Fbxw8^{Gt(pGTOLxf)1Jdec}
• Gene: Fbxw8 Location: Chr5:118203046-118293523 bp, - strand Genetic Position: Chr5, 57.92 cM, cytoband F
• Alliance: Fbxw8^{Gt(RRT057)Byg} page

Mutation origin

• Mutant Cell Line: RRT057
• Germline Transmission: Earliest citation of germline transmission: J:130238
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Hypomorph, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: A genetrap vector containing a beta-gal reporter and a neomycin selection cassette inserted into intron 3 of the gene locus, 2.6 kb downstream of the exon 3. Western analysis on homozygous embryonic fibroblasts indicated protein expression was at 0.8% of the level in wild-type embryonic fibroblasts. Beta gal expression was detected in numerous tissues of homozygotes including cartilage, skeletal muscle, bronchiolar epithelium, and placenta. (J:130238)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRT057	CG887035 ; (MGI Seq Detail)	5' RACE	Chr5:118266875-118267189 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  12 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	26 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fbxw8 Mutation: 30 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:130238 Tsutsumi T, et al., Disruption of the Fbxw8 gene results in pre- and postnatal growth retardation in mice. Mol Cell Biol. 2008 Jan;28(2):743-51
• All: 3 reference(s)