Abcc4^{Gt(RRC212)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Abcc4^{Gt(RRC212)Byg}
• Name: ATP-binding cassette, sub-family C member 4; gene trap RRC212, BayGenomics
• MGI ID: MGI:3526359
• Synonyms: mrp4^-
• Gene: Abcc4 Location: Chr14:118720104-118943631 bp, - strand Genetic Position: Chr14, 62.24 cM
• Alliance: Abcc4^{Gt(RRC212)Byg} page

Mutation origin

• Mutant Cell Line: RRC212
• Germline Transmission: Earliest citation of germline transmission: J:139829
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The gene trap vector, pGT1lxf, inserted between exon 18 and exon 19. RT-PCR and western blot analysis confirm the absence of mTNA and protein expression in homozygous MEFs. (J:139829)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRC212	CC200259 ; (MGI Seq Detail)	5' RACE	Chr14:118834094-118836934 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  114 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Abcc4 Mutation: 151 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:139829 Lin ZP, et al., Disruption of cAMP and prostaglandin E2 transport by multidrug resistance protein 4 deficiency alters cAMP-mediated signaling and nociceptive response. Mol Pharmacol. 2008 Jan;73(1):243-51
• All: 2 reference(s)