Tmprss2^tm1Psn
Targeted Allele Detail

Summary

• Symbol: Tmprss2^tm1Psn
• Name: transmembrane protease, serine 2; targeted mutation 1, Peter Nelson
• MGI ID: MGI:3526414
• Synonyms: Tmprss2^-, Tmprss2^tm1Tsyk
• Gene: Tmprss2 Location: Chr16:97365882-97412395 bp, - strand Genetic Position: Chr16, 57.53 cM, cytoband C2
• Alliance: Tmprss2^tm1Psn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:105558
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace exons 10-13 with a loxP-flanked neo. The deleted exons encode 2 of 3 catalytic residues essential for serine protease activity. Quantitative RT-PCR failed to detect transcript of exons 11 and 13. Mutants expressed only a 20% reduction in transcripts spanning exons 3 and 4 or 5 and 6, however, mutants expressed 80% less transcript of the 3' UTR than wild-type mice. Sequencing revealed that mutant transcripts alternatively splice exon 9 to exon 14. (J:105558)

Expression

In Mice Carrying this Mutation:

23 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tmprss2 Mutation: 55 strains or lines available

References

• Original: J:105558 Kim TS, et al., Phenotypic analysis of mice lacking the Tmprss2-encoded protease. Mol Cell Biol. 2006 Feb;26(3):965-75
• All: 11 reference(s)