Nherf1^tm1Geo
Targeted Allele Detail

Summary

• Symbol: Nherf1^tm1Geo
• Name: NHERF family PDZ scaffold protein 1; targeted mutation 1, Maria-Magdelena Georgescu
• MGI ID: MGI:3526430
• Synonyms: EBP50^-
• Gene: Nherf1 Location: Chr11:115054167-115072007 bp, + strand Genetic Position: Chr11, 80.65 cM, cytoband E2
• Alliance: Nherf1^tm1Geo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95282
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace exons 1-4, which contain most of the coding sequence, with a neo gene lacking the polyadenlylation sequence. RT-PCR and sequencing revealed that exons 5 and 6 were transcribed after the stop codon of the neo mRNA, as part of the 3' UTR. Absence of protein from exons 5 and 6 was further confirmed by Western blot analysis of kidneys from mutant animals. (J:95282)

Expression

In Mice Carrying this Mutation:	5 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nherf1 Mutation: 18 strains or lines available

References

• Original: J:95282 Morales FC, et al., Ezrin-radixin-moesin (ERM)-binding phosphoprotein 50 organizes ERM proteins at the apical membrane of polarized epithelia. Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17705-10
• All: 5 reference(s)