Ihh^tm2Amc
Targeted Allele Detail

Summary

• Symbol: Ihh^tm2Amc
• Name: Indian hedgehog; targeted mutation 2, Andrew P McMahon
• MGI ID: MGI:3526438
• Gene: Ihh Location: Chr1:74984474-74990831 bp, - strand Genetic Position: Chr1, 38.55 cM
• Alliance: Ihh^tm2Amc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99641
• Parent Cell Line: AV3 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Frt-flanked neo-tk cassette with a loxP site was introduced into the first intron and another loxP site was introduced into the 5' leader sequence of the first exon by homologous recombination. The neo-tk cassette was excised by transient transfection of properly targeted ES cells with a flpe-expressing plasmid, leaving exon 1 flanked by loxP sites. (J:99641)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ihh Mutation: 22 strains or lines available

References

• Original: J:99641 Kobayashi T, et al., Indian hedgehog stimulates periarticular chondrocyte differentiation to regulate growth plate length independently of PTHrP. J Clin Invest. 2005 Jul;115(7):1734-42
• All: 1 reference(s)