Flt1^tm2Msh
Targeted Allele Detail

Summary

• Symbol: Flt1^tm2Msh
• Name: FMS-like tyrosine kinase 1; targeted mutation 2, Masabumi Shibuya
• MGI ID: MGI:3527129
• Synonyms: Flt1-TM-TK^-, VEGFR1(TM-TK)^-
• Gene: Flt1 Location: Chr5:147498414-147662821 bp, - strand Genetic Position: Chr5, 87.01 cM, cytoband G
• Alliance: Flt1^tm2Msh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95387
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance gene replaced exon 16, encoding the transmembrane domain. No clear bands were seen for exons downstream of exon 15 in RT-PCR analysis, showing that the protein was truncated at the termination codon in exon 15. Mutant lung tissues demonstrated that the 110-kDa endogenous soluble form was present, as it results from a termination in intron 13. Also present was a new truncated form, approximately 120-kDa, detected with an antiserum raised against the amino acid sequence of exon 15. (J:95387)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Flt1 Mutation: 76 strains or lines available

References

• Original: J:95387 Hiratsuka S, et al., Membrane fixation of vascular endothelial growth factor receptor 1 ligand-binding domain is important for vasculogenesis and angiogenesis in mice. Mol Cell Biol. 2005 Jan;25(1):346-54
• All: 3 reference(s)