Smad2^{tm4(SMADH3)Rob}
Targeted Allele Detail

Summary

• Symbol: Smad2^{tm4(SMADH3)Rob}
• Name: SMAD family member 2; targeted mutation 4, Elizabeth J Robertson
• MGI ID: MGI:3527156
• Synonyms: Smad2^hSmad3ki
• Gene: Smad2 Location: Chr18:76374651-76444034 bp, + strand Genetic Position: Chr18, 51.42 cM
• Alliance: Smad2^{tm4(SMADH3)Rob} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95374
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to subsequently delete exon 2 coding sequence and insert the amino-terminal flag-tagged human SMAD3 cDNA into the locus. A triply repeated SV40 polyA sequence was inserted to ensure transcriptional termination as well as a floxed PGK-neo. Transient cre expression excised the floxed neo. Ribonuclease protection assay probes were designed to confirm expression of the human cDNA, and residual endogenous transcripts were barely detectable in mutant tissues. Recombined transcript expression levels in mutants were comparable to that of endogenous levels in wild-type animals. (J:95374)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Smad2 Mutation: 52 strains or lines available

References

• Original: J:95374 Dunn NR, et al., Mice exclusively expressing the short isoform of Smad2 develop normally and are viable and fertile. Genes Dev. 2005 Jan 1;19(1):152-63
• All: 1 reference(s)