Drd1^tm2Rpa
Targeted Allele Detail

Summary

• Symbol: Drd1^tm2Rpa
• Name: dopamine receptor D1; targeted mutation 1, Richard D Palmiter
• MGI ID: MGI:3527157
• Synonyms: D1R-NR1^m, Drd1a^dnNR1, Drd1a^Grin1-N616R
• Gene: Drd1 Location: Chr13:54205202-54209677 bp, - strand Genetic Position: Chr13, 28.4 cM
• Alliance: Drd1^tm2Rpa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:121750
• Parent Cell Line: v6.4 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Dominant negative)
• Mutation: Insertion
• Mutation details: A cDNA open reading frame encoding a dominant-negative mutant NR1 subunit of the NMDA receptor with an asparagine-to-arginine substitution at amino acid 616 (N616R), a hemagglutinin (HA) epitope tag 27 aa from the N terminus and a polyadenylation signal, followed by a loxP-flanked SV40-neo cassette, has replaced the first ~130 base pairs of the endogenous Drd1a gene. (J:121750)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Drd1 Mutation: 70 strains or lines available

References

• Original: J:121750 Heusner CL, et al., Expression of mutant NMDA receptors in dopamine D1 receptor-containing cells prevents cocaine sensitization and decreases cocaine preference. J Neurosci. 2005 Jul 13;25(28):6651-7
• All: 2 reference(s)