Npy^tm2Rpa
Targeted Allele Detail

Summary

• Symbol: Npy^tm2Rpa
• Name: neuropeptide Y; targeted mutation 2, Richard D Palmiter
• MGI ID: MGI:3527174
• Synonyms: Npy^tet
• Gene: Npy Location: Chr6:49799690-49806487 bp, + strand Genetic Position: Chr6, 24.04 cM, cytoband B3
• Alliance: Npy^tm2Rpa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:104699
• Parent Cell Line: AK18.1 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Tetracycline activator and tet promoter targeted to first exon such that expression can be turned off with doxycycline. The TK-Neo and Ura3 genes from the tetracycline cassette were removed by breeding with Mox2-cre mice. In the absence of doxycyline, there is approximately 4-fold more mRNA and increased protein expression in the brain. Doxycycline treatment for 10 days reduces protein expression by about 50%. (J:104699)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Npy Mutation: 19 strains or lines available

References

• Original: J:104699 Ste Marie L, et al., Modulation of neuropeptide Y expression in adult mice does not affect feeding. Proc Natl Acad Sci U S A. 2005 Dec 20;102(51):18632-7
• All: 3 reference(s)