Rad50^tm1Flv
Targeted Allele Detail

Summary

• Symbol: Rad50^tm1Flv
• Name: RAD50 double strand break repair protein; targeted mutation 1, Richard A Flavell
• MGI ID: MGI:3527268
• Synonyms: RHS7-deficient
• Gene: Rad50 Location: Chr11:53540346-53598146 bp, - strand Genetic Position: Chr11, 31.98 cM, cytoband A5-B1
• Alliance: Rad50^tm1Flv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94870
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted
• Mutation: Intragenic deletion
• Mutation details: The RHS7 site (Rad50 hypersensitive site 7), a 637 bp region located in intron 24, was replaced with a floxed neomycin resistance cassette. The resistance cassette was subsequently removed from the ES cells by transient cre expression. There was no effect on Rad50 expression. (J:94870)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rad50 Mutation: 53 strains or lines available

Notes

RHS7 makes up one component of a locus control region for TH2 cytokines.

References

• Original: J:94870 Lee GR, et al., Hypersensitive site 7 of the TH2 locus control region is essential for expressing TH2 cytokine genes and for long-range intrachromosomal interactions. Nat Immunol. 2005 Jan;6(1):42-8
• All: 4 reference(s)