Tg(ACTB-Twnk)#Suom
Transgene Detail

Summary

• Symbol: Tg(ACTB-Twnk)#Suom
• Name: transgene insertion, Anu Suomalainen
• MGI ID: MGI:3527553
• Synonyms: Tg(ACTB-Peo1)#Suom, Tw⁺
• Transgene: Tg(ACTB-Twnk)#Suom Location: unknown
• Alliance: Tg(ACTB-Twnk)#Suom page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(ACTB-Twnk)#Suom expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Twnk (MGI:2137410)

• Mutation details: The transgene consists of the cDNA and intron 4 of the mouse progressive external ophthalmoplegia 1 gene under the control of the human beta actin promoter. RT-PCR analysis of muscle and heart tissues detected transgene expression. Southern blot analysis estimated founder line A contains 3 to 4 copies of the transgene, and founder line B contains 1 to 2 copies of the transgene. The A mice displayed a 6 fold increase of Twinkle mRNA in skeletal muscle and 8.4 fold increase in heart, whereas the B mice had a 1.5 fold and 4.2 fold increase in the respective tissues. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:95313)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

This is a representative record representing founder lines A and B.

References

• Original: J:95313 Tyynismaa H, et al., Twinkle helicase is essential for mtDNA maintenance and regulates mtDNA copy number. Hum Mol Genet. 2004 Dec 15;13(24):3219-27
• All: 10 reference(s)