Tgm2^tm1.1Rmgr
Targeted Allele Detail

Summary

• Symbol: Tgm2^tm1.1Rmgr
• Name: transglutaminase 2, C polypeptide; targeted mutation 1.1, Robert M Graham
• MGI ID: MGI:3527996
• Synonyms: TG2^-, TGase 2-, Tgm2^-, tTg^-
• Gene: Tgm2 Location: Chr2:157958325-157988312 bp, - strand Genetic Position: Chr2, 78.72 cM
• Alliance: Tgm2^tm1.1Rmgr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95130
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exons 6-8 were excised following cre-mediated excision. This sequence encoded the TGase catalytic core domain. Northern blot and RT-PCR both confirmed absence of transcript in mutant hearts. Lack of protein was demonstrated in mutant liver membranes and heart cytosol by Western blot analysis. (J:95130)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Tgm2^tm1.1Rmgr/Tgm2^tm1.1Rmgr	129(B6)-Tgm2^tm1.1Rmgr
hm2	Tgm2^tm1.1Rmgr/Tgm2^tm1.1Rmgr	B6.129S1-Tgm2^tm1.1Rmgr
hm3	Tgm2^tm1.1Rmgr/Tgm2^tm1.1Rmgr	involves: 129S1/SvImJ * C57BL/6
cx4	Tgm2^tm1.1Rmgr/Tgm2^tm1.1Rmgr Tg(HDexon1)62Gpb/0	involves: 129X1/SvJ * C57BL/6 * CBA
cx5	Tgm2^tm1.1Rmgr/Tgm2^+ Tg(HDexon1)62Gpb/0	involves: 129X1/SvJ * C57BL/6 * CBA

Phenotypes:

Affected Systems	hm1	hm2	hm3	cx4	cx5
show or hide all annotated terms
behavior/neurological				√
impaired coordination				√
cellular			√
abnormal cell adhesion			√
growth/size/body	N	√
growth/size/body region phenotype	N
increased body weight		√
hematopoietic system			√
abnormal T cell physiology			√
homeostasis/metabolism	N	N
homeostasis/metabolism phenotype	N	N
immune system			√
abnormal T cell physiology			√
mortality/aging				√
extended life span				√
nervous system				√	√
abnormal striatum morphology				√	√
abnormal cerebral cortex morphology				√	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	1 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Tgm2 Mutation: 49 strains or lines available

References

• Original: J:95130 Nanda N, et al., Targeted inactivation of Gh/tissue transglutaminase II. J Biol Chem. 2001 Jun 8;276(23):20673-8
• All: 27 reference(s)