Drd5^tm1Sib
Targeted Allele Detail

Summary

• Symbol: Drd5^tm1Sib
• Name: dopamine receptor D5; targeted mutation 1, David R Sibley
• MGI ID: MGI:3528074
• Synonyms: D5^-
• Gene: Drd5 Location: Chr5:38476742-38479868 bp, + strand Genetic Position: Chr5, 20.4 cM
• Alliance: Drd5^tm1Sib page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95908
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neo was inserted in reverse orientation to disrupt the reading frame within the coding region. A stop codon was engineered into the proximal neo linker to prematurely truncate the transcript subsequent to Gly-190 in the second extracellular loop. RT-PCR verified presence of the recombinant transcript in mutant brains. Immunohistochemistry using peptides from the region downstream of the stop codon showed that mutants did not produce the corresponding protein. (J:95908)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Drd5 Mutation: 39 strains or lines available

References

• Original: J:95908 Hollon TR, et al., Mice lacking D5 dopamine receptors have increased sympathetic tone and are hypertensive. J Neurosci. 2002 Dec 15;22(24):10801-10
• All: 24 reference(s)