Lp1^DBA/1J
QTL Variant Detail

Summary

• QTL variant: Lp1^DBA/1J
• Name: lymphocyte proliferation 1; DBA/1J
• MGI ID: MGI:3528168
• QTL: Lp1 Location: Chr16:6542979-65466552 bp Genetic Position: Chr16, cM position of peak correlated region/allele: 29.93 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: DBA/1J

Variant description

• Allele Type: QTL

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:94347

Linkage analysis was performed on 290 animals from a (DBA/1J x FVB/N)F2 intercross to identify QTLs associated with arthritis traits. 126 polymorphic markers with 92% coverage of the mouse genome at an average spacing of 11.5 cM were used for the genome scan. Parental strain DBA/1J is susceptible to collagen-induced arthritis whereas parental strain FVB/N is resistant. Experimental animals were immunized with bovine collagen at 8-12 weeks of age to induce arthritis.

QTLs Cia24 and Cia25 were detected on mouse Chromosome 2. Both Cia24 and Cia25 showed linkage to D2Mit81 at 13.5 cM. Cia24 is associated with arthritis severity (LOD=12.1) and Cia25 is associated with onset of phenotype (LOD=10.6). The QTL interval is flanked by markers D2Mit81 and D2Mit367. A potential candidate gene for Cia24 and Cia25 is Hc at 23.5 cM. Parental strain FVB/N has a 2 bp deletion in the Hc exon 5 region, which results in Hc deficiency. The Hc deficiency phenotype cosegregates with decreased arthritis severity in (DBA/1J x FVB/N)F2 animals.

Novel QTL Trmq3 (T cell ratio modifier QTL 3) mapped to 65 cM on mouse Chromosome 2 near D2Mit398. This locus is linked to CD4:CD8 ratio (LOD=5). FVB/N-derived alleles at Trmq3 confer increased CD4:CD8 ratio. The QTL interval of Trmq3 isflanked by markers D2Mit61 and D2Mit395. Potential candidate genes for Trmq3 are Notch1 (15 cM) and Bcl2l1 (87.5 cM). Trmq3 maps near the previously identified QTL Trmq2 (52 cM).

Novel QTL Cia27 (collagen induced arthritis 27)mapped to 60 cM on mouse Chromosome 5 near D5Mit277. This locus is associated with collagen-specific IgG2a antibody levels (LOD=4.3). The QTL interval is flanked by markers D5Mit201 and D5Mit425. DBA/1J-derived alleles at Cia27 confer increased IgG2a antibodies. Cia27 and Cia29 show evidence of interaction in affecting IgG2a levels (LOD=10.34).

Novel QTL Cia28 (collagen induced arthritis 28) mapped to 52 cM on mouse Chromosome 13 near D13Mit144. This locus is linked to collagen-specific IgG1 antibody levels (LOD=3.2). The QTL interval of Cia28 is flanked by markers D13Mit13 and D13Mit144. Cia28 shows evidence of interaction with Cia9 on mouse Chromosome 1 (D1Mit111) in affecting IgG1 levels (LOD=9.12).

Novel QTL Cia29 (collagen induced arthritis 29) mapped to 52 cM on mouse Chromosome 10 near D10Mit96. This locus is linked to collagen-specific IgG1 antibody levels (LOD=3.6). The QTL interval of Cia29 is flanked by markers D10Mit261 and D10Mit14. Previously identified arthritis QTL Cia8 (40.4 cM) mapsnear Cia29.

Novel QTL Lp1 (lymphocyte proliferation 1) mapped to 31 cM on mouse Chromosome 16 near D16Mit138. This locus is linked to lymphocyte proliferation (LOD=3.8). The QTL interval for Lp1 is flanked by markers D16Mit160 and D16Mit139. Lp1 maps near previously identified QTL Eae11 (41 cM).

References

• Original: J:94347 Bauer K, et al., Identification of new quantitative trait loci in mice with collagen-induced arthritis. Arthritis Rheum. 2004 Nov;50(11):3721-8
• All: 1 reference(s)