Tg(TcraCDC35)1Alhn
Transgene Detail

Summary

• Symbol: Tg(TcraCDC35)1Alhn
• Name: transgene insertion 1, Agnes Lehuen
• MGI ID: MGI:3528229
• Synonyms: A8, Valpha8 Tg
• Transgene: Tg(TcraCDC35)1Alhn Location: unknown
• Alliance: Tg(TcraCDC35)1Alhn page

Transgene origin

• Strain of Origin: NOD

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(TcraCDC35)1Alhn expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)

• Mutation details: The transgene contains the V-J exon from the rearranged Tcra gene of the (C3H/HeJ x DBA/2J)F1-derived, IA-d-restricted, rabbit immunoglobulin-specific CDC35 T-lymphocyte cell line. This V-J exon, in which Tcra-V8 is joined to Tcra-J37, was inserted into the 2B4alphaEH vector between a 2-kb germline DNA fragment from the Tcra-V11 5' flanking region, which contains the natural Tcra-V promoter, and a chimeric sequence comprising: a 681-bp fragment containing the immunoglobulin heavy chain enhancer; approximately 100 bp from the 5' end of the Tcra-J-C intron; and a 9.8-kb germline DNA fragment including 6 kb of Tcra-C 5'-flanking DNA, all of the Tcra constant-region exons, and 0.5 kb of 3'-flanking DNA. (J:93798)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:93559 Mars LT, et al., Cutting edge: V alpha 14-J alpha 281 NKT cells naturally regulate experimental autoimmune encephalomyelitis in nonobese diabetic mice. J Immunol. 2002 Jun 15;168(12):6007-11
• All: 3 reference(s)