En2^{tm4(cre/ERT2)Alj}
Targeted Allele Detail

Summary

• Symbol: En2^{tm4(cre/ERT2)Alj}
• Name: engrailed 2; targeted mutation 4, Alexandra L Joyner
• MGI ID: MGI:3528247
• Synonyms: En2-CreER^T2, En2^tm4(cre)Alj
• Gene: En2 Location: Chr5:28370692-28377164 bp, + strand Genetic Position: Chr5, 13.94 cM
• Alliance: En2^{tm4(cre/ERT2)Alj} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95823
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• En2^{tm4(cre/ERT2)Alj} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	En2 (MGI:95390)

• Mutation details: A tamoxifen dependent form of cre recombinase (CreER) and a loxP-flanked neo in reverse orientation were inserted into the 5' UTR. The neo was removed in vivo via Cre-mediated recombination. (J:95823)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	En2 (mouse) Summary of all recombinase alleles driven by En2. Comparative matrix view of recombinase activities Alliance mouse En2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any En2 Mutation: 104 strains or lines available

References

• Original: J:95823 Sgaier SK, et al., Morphogenetic and cellular movements that shape the mouse cerebellum; insights from genetic fate mapping. Neuron. 2005 Jan 6;45(1):27-40
• All: 3 reference(s)