En1^{tm7(cre/ESR1)Alj}
Targeted Allele Detail

Summary

• Symbol: En1^{tm7(cre/ESR1)Alj}
• Name: engrailed 1; targeted mutation 7, Alexandra L Joyner
• MGI ID: MGI:3528251
• Synonyms: En1-CreER^T1, En1-CreERT1, En1^tm7(cre)Alj, Engrailed1-Cre
• Gene: En1 Location: Chr1:120530246-120535719 bp, + strand Genetic Position: Chr1, 52.74 cM, cytoband C2-qter
• Alliance: En1^{tm7(cre/ESR1)Alj} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95823
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• En1^{tm7(cre/ESR1)Alj} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	En1 (MGI:95389)

• Mutation details: A tamoxifen dependent form of cre recombinase (CreER) and a loxP-flanked neo in reverse orientation were inserted into the locus. The neo was removed in vivo via Cre-mediated recombination. (J:95823)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	En1 (mouse) Summary of all recombinase alleles driven by En1. Comparative matrix view of recombinase activities Alliance mouse En1 gene page

Expression

In Mice Carrying this Mutation:

1 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any En1 Mutation: 34 strains or lines available

References

• Original: J:95823 Sgaier SK, et al., Morphogenetic and cellular movements that shape the mouse cerebellum; insights from genetic fate mapping. Neuron. 2005 Jan 6;45(1):27-40
• All: 14 reference(s)