Trp53^tm3.1Glo
Targeted Allele Detail

Summary

• Symbol: Trp53^tm3.1Glo
• Name: transformation related protein 53; targeted mutation 3.1, Guillermina Lozano
• MGI ID: MGI:3528279
• Synonyms: p53^515A, p53^H, p53^R172H
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm3.1Glo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95318
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Dominant negative, Humanized sequence)
• Mutations: Insertion, Single point mutation
• Mutation details: Arginine codon 172 (CGC) was changed to histidine (CAC) (c.515G>A, p.R172H, equivalent to p.R175H in humans) and a loxP site flanked neomycin resistance gene cassette was inserted into intron 4. The neo cassette was excised via Cre-mediated recombination. Western blot analysis of homozygous MEFs detected mutant protein. (J:95318)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Trp53^tm3.1Glo

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 240 strains or lines available

References

• Original: J:95318 Lang GA, et al., Gain of function of a p53 hot spot mutation in a mouse model of Li-Fraumeni syndrome. Cell. 2004 Dec 17;119(6):861-72
• All: 35 reference(s)