Aqp11^tm1Lex
Targeted Allele Detail

Summary

• Symbol: Aqp11^tm1Lex
• Name: aquaporin 11; targeted mutation 1, Lexicon Genetics
• MGI ID: MGI:3528447
• Synonyms: Aqp11-
• Gene: Aqp11 Location: Chr7:97375586-97387454 bp, - strand Genetic Position: Chr7, 53.57 cM
• Alliance: Aqp11^tm1Lex page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100522
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting construct was designed to delete exons 2 and 3, corresponding to the second NPA box of the locus, and replace them with an IRES-beta-gal-MC1-neo cassette. Northern blot of mutants demonstrated lack of transcript in kidney and liver samples. Western blot and immunohistochemistry confirmed absence of protein in mutant kidney samples. (J:100522)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Aqp11 Mutation: 16 strains or lines available

References

• Original: J:100522 Morishita Y, et al., Disruption of aquaporin-11 produces polycystic kidneys following vacuolization of the proximal tubule. Mol Cell Biol. 2005 Sep;25(17):7770-9
• All: 6 reference(s)