Tg(HLA-DRB1)31Dmz
Transgene Detail

Summary

• Symbol: Tg(HLA-DRB1)31Dmz
• Name: transgene insertion 31, Dennis M Zaller
• MGI ID: MGI:3529465
• Synonyms: DR4Dw4, DRB1*0401, HLA-DR4, HLA-DR4 (DRB1*0401)
• Transgene: Tg(HLA-DRB1)31Dmz Location: unknown
• Alliance: Tg(HLA-DRB1)31Dmz page

Transgene origin

• Strain of Origin: (C57BL/6J x SJL/J)F2

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(HLA-DRB1)31Dmz expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  RBM45 (129831)

  Rbm45

  (MGI:2387367)

• Mutation details: A chimeric MHC class II complex was created by fusing the human peptide binding domains of class II alpha and beta chains to the CD4 binding domains of murine class II alpha or beta chains. The was done by the construction of two separate transgene constructs. The alpha1 and beta1 domains isolated from human DRB1*0401 (DR4Dw4) replaced the corresponding alpha1 (aa 1-85) and beta1 (aa 1-96) domains of the H2-Ea and H2-Eb1 genes that make up mouse I-E^d. The native murine promoters were left intact. These transgene constructs were co-injected into fertilized eggs. Flow cytometry analysis indicates that virtually all B-cells and macrophages co-express the chimeric MHC class II molecules. The MHC class II chimeric molecules can be physiologically regulated as there is a 3-4 fold increase in MHC II molecules when splenic B-cells are cultured overnight with IL-4. (J:96129)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:96129 Woods A, et al., Human major histocompatibility complex class II-restricted T cell responses in transgenic mice. J Exp Med. 1994 Jul 1;180(1):173-81
• All: 24 reference(s)