Pik3c2a^{Gt(OST288105)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Pik3c2a^{Gt(OST288105)Lex}
• Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha; gene trap OST288105, Lexicon Genetics
• MGI ID: MGI:3529840
• Synonyms: Pik3c2a^{Gt(neo)268Lex}
• Gene: Pik3c2a Location: Chr7:115936500-116042684 bp, - strand Genetic Position: Chr7, 61.62 cM
• Alliance: Pik3c2a^{Gt(OST288105)Lex} page

Mutation origin

• Mutant Cell Line: OST288105
• Germline Transmission: Earliest citation of germline transmission: J:94338
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: The gene trap vector inserted into intron 27. The expression of a truncated protein product, missing the 267 amino C-terminal amino acids, was confirmed by western blot analysis. Reduced enzymatic activity was confirmed in the liver and kidney. (J:169710)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST288105	CG608214 ; (MGI Seq Detail)	3' RACE	Chr7:115941446-115945475 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  9 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Pik3c2a^Gt(OST288105)Lex/Pik3c2a^Gt(OST288105)Lex	involves: 129S5/SvEvBrd * C57BL/6J

Phenotypes:

Affected Systems	hm1
show or hide all annotated terms
adipose tissue	√
decreased total body fat amount	√
growth/size/body	√
decreased lean body mass	√
decreased body size	√
decreased body weight	√
postnatal growth retardation	√
enlarged spleen	√
hematopoietic system	√
enlarged spleen	√
decreased erythrocyte cell number	√
decreased hematocrit	√
decreased hemoglobin content	√
thrombocytosis	√
increased mean platelet volume	√
increased leukocyte cell number	√
increased granulocyte number	√
increased neutrophil cell number	√
increased B cell number	√
increased CD4-positive, alpha-beta T cell number	√
increased CD8-positive, alpha-beta T cell number	√
increased monocyte cell number	√
increased IgA level	√
increased IgG level	√
increased IgM level	√
homeostasis/metabolism	√
abnormal blood homeostasis	√
increased circulating creatinine level	√
increased blood urea nitrogen level	√
decreased circulating bilirubin level	√
increased circulating cholesterol level	√
increased circulating phosphate level	√
increased circulating potassium level	√
increased circulating alkaline phosphatase level	√
increased urine protein level	√
immune system	√
immune system phenotype	N
enlarged spleen	√
increased leukocyte cell number	√
increased granulocyte number	√
increased neutrophil cell number	√
increased B cell number	√
increased CD4-positive, alpha-beta T cell number	√
increased CD8-positive, alpha-beta T cell number	√
increased monocyte cell number	√
increased IgA level	√
increased IgG level	√
increased IgM level	√
increased anti-double stranded DNA antibody level	√
glomerulonephritis	√
mortality/aging	√
premature death	√
renal/urinary system	√
increased urine protein level	√
abnormal kidney morphology	√
abnormal podocyte morphology	√
podocyte foot process effacement	√
abnormal renal glomerulus morphology	√
glomerulonephritis	√
expanded mesangial matrix	√
glomerulosclerosis	√
small kidney	√
pale kidney	√
decreased creatinine clearance	√
kidney failure	√
polyuria	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	1 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pik3c2a Mutation: 100 strains or lines available

References

• Original: J:94338 NIH Mouse Knockout Inventory, Information obtained from the NIH Mouse Knockout Inventory. MGI Direct Data Submission. 2004
• All: 5 reference(s)