Dlc1^tm1Lec
Targeted Allele Detail

Summary

• Symbol: Dlc1^tm1Lec
• Name: deleted in liver cancer 1; targeted mutation 1, Laboratory of Experimental Carcinogenesis
• MGI ID: MGI:3529997
• Synonyms: DLC-1^-
• Gene: Dlc1 Location: Chr8:37034905-37420297 bp, - strand Genetic Position: Chr8, 23.05 cM, cytoband A4-B2
• Alliance: Dlc1^tm1Lec page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:96194
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 5, encoding a serine-rich domain, was replaced with a neomycin resistance gene, causing a frame shift that resulted in a premature stop codon. The synthesized truncated protein should contain only the first 77 amino acids, which encode the SAM domain and 23 novel residues due to the reading frame shift, however, Northern blots of heterozygous animals did not demonstrate such a truncated transcript. (J:96194)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dlc1 Mutation: 227 strains or lines available

References

• Original: J:96194 Durkin ME, et al., DLC-1, a Rho GTPase-activating protein with tumor suppressor function, is essential for embryonic development. FEBS Lett. 2005 Feb 14;579(5):1191-6
• All: 2 reference(s)