Dtx1^tm1Crey
Targeted Allele Detail

Summary

• Symbol: Dtx1^tm1Crey
• Name: deltex 1, E3 ubiquitin ligase; targeted mutation 1, Claude-Agnes Reynaud
• MGI ID: MGI:3530026
• Synonyms: Deltex-1^delta
• Gene: Dtx1 Location: Chr5:120818267-120849992 bp, - strand Genetic Position: Chr5, 60.64 cM
• Alliance: Dtx1^tm1Crey page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95986
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP-flanked neo replaced exons 4-10 (G321 to A626), which encode the C-terminal half of the protein that includes the RING finger domain. Northern blot showed the presence of three new transcripts. The longest form putatively corresponds to a partially spliced mRNA stabilized by the polyA signal of neo. The shortest form was suggested to be created by splicing of the donor site of exon 3 to a cryptic acceptor site in the neo, which created an in-frame stop codon 7 amino acids downstream. It could not be determined if a truncated protein without the RING finger domain was expressed. (J:95986)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dtx1 Mutation: 36 strains or lines available

References

• Original: J:95986 Storck S, et al., Normal immune system development in mice lacking the Deltex-1 RING finger domain. Mol Cell Biol. 2005 Feb;25(4):1437-45
• All: 1 reference(s)