Otos^tm1Cham
Targeted Allele Detail

Summary

• Symbol: Otos^tm1Cham
• Name: otospiralin; targeted mutation 1, Christian P Hamel
• MGI ID: MGI:3530083
• Synonyms: Otos^-
• Gene: Otos Location: Chr1:92571940-92576563 bp, - strand Genetic Position: Chr1, 46.55 cM
• Alliance: Otos^tm1Cham page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:96000
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A lacZneo was fused in frame after nucleotide 10 of the ATG in exon 2, and replaced part of exon 2 and all of exons 3 and 4. RT-PCR and Western blot analysis of the inner ear of mutants demonstrated the lack of transcript and protein, respectively. (J:96000)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Otos Mutation: 11 strains or lines available

References

• Original: J:96000 Delprat B, et al., Deafness and cochlear fibrocyte alterations in mice deficient for the inner ear protein otospiralin. Mol Cell Biol. 2005 Jan;25(2):847-53
• All: 1 reference(s)