Nfib^tm1Rmg
Targeted Allele Detail

Summary

• Symbol: Nfib^tm1Rmg
• Name: nuclear factor I/B; targeted mutation 1, Richard M Gronostajski
• MGI ID: MGI:3530139
• Synonyms: Nfib^-
• Gene: Nfib Location: Chr4:82208410-82424988 bp, - strand Genetic Position: Chr4, 38.4 cM, cytoband C4-C6
• Alliance: Nfib^tm1Rmg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:96006
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An NSL-Betagal-pA cassette and a PGK-neo-bGHpA cassette replaced 700 bp, including all but 9 bp of exon 2. Exon 2 encodes both the DNA-binding and dimerization active sites. RT-PCR of mutant brain, liver and lung samples indicated the absence of wild type transcript. An aberrantly spliced 152-bp amplicon was present in mutants in low abundance, putatively containing exon 1 spliced directly to exon 3 that would encode a 35 amino acid peptide terminating in exon 4. (J:96006)

Expression

In Mice Carrying this Mutation:	32 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nfib Mutation: 91 strains or lines available

References

• Original: J:96006 Steele-Perkins G, et al., The transcription factor gene Nfib is essential for both lung maturation and brain development. Mol Cell Biol. 2005 Jan;25(2):685-98
• All: 19 reference(s)