Nkd1^tm1Tko
Targeted Allele Detail

Summary

• Symbol: Nkd1^tm1Tko
• Name: naked cuticle 1; targeted mutation 1, Makoto M Taketo
• MGI ID: MGI:3530367
• Synonyms: Nkd1^-
• Gene: Nkd1 Location: Chr8:89247982-89321512 bp, + strand Genetic Position: Chr8, 43.36 cM, cytoband C4
• Alliance: Nkd1^tm1Tko page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95901
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 5 and 6, containing 60 amino acids comprising most of the EF-hand-containing oxygen donation and ion-binding domains, were replaced with a PGK-neo. Exons 4 and 7 were spliced together and resulted in a mutant transcript and protein lacking exons 5 and 6, as shown by Northern blot analysis, RT-PCR and Western blot. (J:95901)

Expression

In Mice Carrying this Mutation:	32 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nkd1 Mutation: 17 strains or lines available

References

• Original: J:95901 Li Q, et al., A targeted mutation of Nkd1 impairs mouse spermatogenesis. J Biol Chem. 2005 Jan 28;280(4):2831-9
• All: 1 reference(s)