Aph1c^tm1.1Bdes
Targeted Allele Detail

Summary

• Symbol: Aph1c^tm1.1Bdes
• Name: aph1 homolog C, gamma secretase subunit; targeted mutation 1.1, Bart De Strooper
• MGI ID: MGI:3530442
• Synonyms: Aph1c^-
• Gene: Aph1c Location: Chr9:66722276-66741953 bp, - strand Genetic Position: Chr9, 36.15 cM, cytoband D
• Alliance: Aph1c^tm1.1Bdes page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:96106
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Cre mediated recombination excised exons 3 and 4 from Aph1c^tm1Bdes, resulting in deletion of exons 3 and 4 and a frame shift in the ORF, subsequently deleting amino acids from 96 on. RT-PCR and Western blot of mutant MEFs demonstrated the lack of expression of mRNA or protein, respectively. (J:96106)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aph1c Mutation: 20 strains or lines available

References

• Original: J:96106 Serneels L, et al., Differential contribution of the three Aph1 genes to gamma-secretase activity in vivo. Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1719-24
• All: 4 reference(s)