Lama1^tm1Ekb
Targeted Allele Detail

Summary

• Symbol: Lama1^tm1Ekb
• Name: laminin, alpha 1; targeted mutation 1, Peter Ekblom
• MGI ID: MGI:3530455
• Synonyms: AlphaILG4-5^-
• Gene: Lama1 Location: Chr17:68004254-68129642 bp, + strand Genetic Position: Chr17, 38.8 cM
• Alliance: Lama1^tm1Ekb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:96100
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: The 7 exons encoding the alphaLm globular (LG) 5 region were removed by fusion of the DraIII and SmaI restriction sites, creating an in-frame deletion. A neo-HSV-tk flanked by loxP sites was inserted downstream of the stop codon and polyA tail. The last 341 residues of the C terminus were deleted and the linker region between alpha1LG3 and alpha1LG4 and a short sequence of the first Beta-strand of the alpha1LG4 domain were left intact. (J:96100)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lama1 Mutation: 186 strains or lines available

References

• Original: J:96100 Scheele S, et al., Laminin alpha1 globular domains 4-5 induce fetal development but are not vital for embryonic basement membrane assembly. Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1502-6
• All: 1 reference(s)