Lim2^{Gt(OST98930)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Lim2^{Gt(OST98930)Lex}
• Name: lens intrinsic membrane protein 2; gene trap OST98930, Lexicon Genetics
• MGI ID: MGI:3530610
• Synonyms: Lim2^Gt, Lim2^{Gt(neo)501Lex}
• Gene: Lim2 Location: Chr7:43079523-43085420 bp, + strand Genetic Position: Chr7, 28.25 cM
• Alliance: Lim2^{Gt(OST98930)Lex} page

Mutation origin

• Mutant Cell Line: OST98930
• Germline Transmission: Earliest citation of germline transmission: J:123284
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: The VICTR48 gene trap vector containing a splice acceptor, neo, polyA signal, PGK-puro and splice donor inserted into intron 3. RT-PCR and Northern blot confirmed successful interruption of transcript. (J:123284)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST98930	CG524671 ; (MGI Seq Detail)	3' RACE	Chr7:43084727-43085306 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  1 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lim2 Mutation: 15 strains or lines available

References

• Original: J:123284 Shiels A, et al., Refractive defects and cataracts in mice lacking lens intrinsic membrane protein-2. Invest Ophthalmol Vis Sci. 2007 Feb;48(2):500-8
• All: 4 reference(s)