Isl1^{tm1(cre/Esr1*)Krc}
Targeted Allele Detail

Summary

• Symbol: Isl1^{tm1(cre/Esr1*)Krc}
• Name: ISL1 transcription factor, LIM/homeodomain; targeted mutation 1, Kenneth R Chien
• MGI ID: MGI:3530632
• Synonyms: isl1-MCM, isl1-mER-Cre-mER, isl1-MerCreMer, Isl1^{tm1(cre/Esr1)Krc}, MerCreMer
• Gene: Isl1 Location: Chr13:116434817-116446225 bp, - strand Genetic Position: Chr13, 64.87 cM, cytoband D2.3
• Alliance: Isl1^{tm1(cre/Esr1*)Krc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:96092
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Isl1^{tm1(cre/Esr1*)Krc} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Isl1 (MGI:101791)

• Mutation details: A targeting cassette containing a tamoxifen-dependent cre flanked by two mutated mouse estrogen receptors that sequester cre to the cytoplasm, followed by an FRT-flanked neo was inserted into the Isl1 locus by homologous recombination. Flp-mediated recombination removed the FRT-flanked neo cassette. A short segment of coding region containing the endogenous ATG codon was deleted. (J:96092, J:156167)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Isl1 (mouse) Summary of all recombinase alleles driven by Isl1. Comparative matrix view of recombinase activities Alliance mouse Isl1 gene page

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Isl1 Mutation: 36 strains or lines available

References

• Original: J:96092 Laugwitz KL, et al., Postnatal isl1+ cardioblasts enter fully differentiated cardiomyocyte lineages. Nature. 2005 Feb 10;433(7026):647-53
• All: 27 reference(s)