Trp53^tm1Att
Targeted Allele Detail

Summary

• Symbol: Trp53^tm1Att
• Name: transformation related protein 53; targeted mutation 1, Laura D Attardi
• MGI ID: MGI:3573778
• Synonyms: Trp53^LSL-25,26, Trp53^LSL-QS
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm1Att page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:96433
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutation: Insertion
• Mutation details: A knock in strategy was used to introduce a loxP-Stop-loxP transcriptional stop element upstream of exon 2 and p.L25Q and p.W26S substitutions into exon 2 (TTATGG>CAATCG). Northern and Western blots of mutant MEFs treated with adeno-virus cre demonstrated that transcript and protein were expressed, respectively. No expression is seen without Cre expression. (J:96433)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 240 strains or lines available

References

• Original: J:96433 Johnson TM, et al., The p53QS transactivation-deficient mutant shows stress-specific apoptotic activity and induces embryonic lethality. Nat Genet. 2005 Feb;37(2):145-52
• All: 7 reference(s)