Itga10^tm1Ref
Targeted Allele Detail

Summary

• Symbol: Itga10^tm1Ref
• Name: integrin, alpha 10; targeted mutation 1, Reinhard Fassler
• MGI ID: MGI:3574026
• Gene: Itga10 Location: Chr3:96552900-96571835 bp, + strand Genetic Position: Chr3, 41.93 cM, cytoband F2.2
• Alliance: Itga10^tm1Ref page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:96524
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An EGFP and loxP flanked neo were inserted to replace exon 1. RT-PCR of hearts from mutant mice demonstrated the lack of transcript. Loss of protein was further confirmed by Western blot of chondrocyte lysates and immunostaining of newborn knee cartilage. (J:96524)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itga10 Mutation: 75 strains or lines available

References

• Original: J:96524 Bengtsson T, et al., Loss of {alpha}10{beta}1 integrin expression leads to moderate dysfunction of growth plate chondrocytes. J Cell Sci. 2005 Mar 1;118(Pt 5):929-36
• All: 1 reference(s)